A method of low-coherence dynamic light scattering is used to study the diffusion properties of Brownian motion particles in concentrated colloidal suspensions. Based on the single scattering theory, considering the variation of refractive index of suspensions with the volume fraction, the effective diffusion coefficients are obtained by analyzing the single scattering spectra that are measured by using low-coherence dynamic light scattering method. The effective coefficients for three different particles are investigated as a function of volume fractions. As a result, the diffusion properties of Brownian particles are influenced by hydrodynamic interactions and statistical structure factor of particles. The diffusion coefficient decreases with increase of the volume fraction. In the volume fraction range from 0.01 to 0.2, the measured effective diffusion coefficients are in good agreement with the theoretical values by considering the Percus-Yevick and Cohen-de Schepper approximations. It is confirmed that the low-coherence dynamic light scattering technique can be used to measure the diffusion properties of Brownian particles with different volume fractions.