The far-field super-resolution imaging is a hot spot in the research.It is found that the microsphere played an important role in the far-field imaging. We design an experiment to realize de-aggregation of microsphere, and theoretically study imaging characteristics of single microsphere. According to the experimental research, we can double the resolution of far-field microscope by utilizing a specific scale of silica microsphere. By using the Nikon microscope with the halide lamp under the silica microsphere and relevant enhanced-medium, we double the imaging effect of a 1 200 line optical grating,and it shows the far-field imaging efficiency of microsphere.