Potato is the fourth largest food crop in China, which has a comprehensive and reasonable nutrient composition and is easy to digest and absorb. Potato tuber is rich in phenolic substances such as chlorogenic acid, which has many health functions such as anti-oxidation, disease resistance, anti-cancer and lipid-lowering. Since chlorogenic acid is closely related to potato flavor, nutrition and resistance quality, systematic determination and analysis of chlorogenic acid content and distribution in different tissues of potato will provide a theoretical basis and reference for improving crop quality. The common cultivars of potato are autotetraploid, so it is difficult to improve potato varieties by cross breeding. While the diploid potato has more advantages in breeding and genome research due to its simple genetic background compared with the tetraploid potato. In this paper, natural variation population (C series) and self-cross separation of F2 population (151 series and RH series) of diploid potatoes were used. The content of chlorogenic acid in different tissues was determined by UV-Vis and the results were statistically analyzed by SPSS16.0. The results showed that, among the 131 samples selected, the chlorogenic acid content in potato leaves of series C is significantly higher than 151 series and RH series. The highest chlorogenic acid content in potato leaves of series C is 12 022.25 mg·kg^-1 FW, the highest chlorogenic acid content in potato leaves of series 151 is 7 685.41 mg·kg^-1 FW and the highest chlorogenic acid content in potato leaves of series RH is 5 486.46 mg·kg^-1 FW. However, the uniformity and stability of C series are lower than 151 series and RH series. The content of chlorogenic acid in potato skin and flesh of C series (181 samples) was further determined and analyzed. The content of chlorogenic acid in potato skin is significantly higher than flesh. The highest content of chlorogenic acid in potato skin is 19 679.59 mg·kg^-1 DW, while the highest content of chlorogenic acid in potato flesh is 2 927.52 mg·kg^-1 DW. On the whole, the content of chlorogenic acid in potato flesh increased with the skin, but there is no linear relation ship. The content of chlorogenic acid in diploid potato was determined and analyzed, which provided a theoretical basis for the application of spectroscopic analysis in the study and breeding new potato varieties with a high content of chlorogenic acid and good flavor. At the same time, the results of the spectrometric analysis are of great significance in guiding breeding, determination and analysis of natural active substances and agricultural genome research, and also provide research and technical application basis for UV-Vis spectroscopy application in national potato program “Upotato Plan”, international agricultural genome program and intelligent agriculture.