In order to study the possibility of the HAP-QD conjugate of hydroxyapatite (HAP) nanoparticles and cadmium selenide quantum dots (CdSe QD) as a biological nano-temperature probe, HAP nanoparticles are chemically coupled with CdSe QD to obtain HAP-QD conjugate, and further study the temperature characteristics of HAP-QD fluorescence spectrum. First, the surface of HAP is modified by silane coupling agent KH550. Then, under the action of coupling activator, the CdSe QD with a carboxyl group modified on the surface and HAP with an amino group modified on the surface are covalently coupled to obtain a HAP-QD conjugate. Finally, the fluorescence spectra of CdSe QD and HAP-QD are measured at the temperature range of 298--318 K. The experimental results show that the fluorescence peak position of HAP-QD demonstrates a redshift and has a good linear relationship with the increase of temperature.