Presently, the study of dye-sensitized solar cells (DSSCs) has become one of the focus of solar cells research. Owing to low cost, easy fabrication process, and high power conversion efficiency (PCE), the DSSCs with the nano-crystalline porous TiO₂ film as photo-anode by the preparation of screen printing are widely concerned. It is very important to optimize the screen printing process. This method is very effective to study the effect of the screen printing process on the photovoltaic performance of DSSCs. The TiO₂ were prepared by sol-gel method, and the porous structure of TiO₂ thin film observed by SEM has a high specific surface area, which is conducive to the adsorption of dye molecules and improving the solar absorption rate. The screen printing TiO₂ film after high-temperature sintering shows a narrow diffraction peak of anatase structure, which implies that TiO₂ particles have been fully crystallized. The increase of mesh number from 100 to 300 leads to the decrease of diameter and the increase of TiO₂ film density, making the diffraction peak of XRD enhanced. However, the increases from 300 to 400 mesh decrease TiO₂ colloids passing through the mesh due to the small mesh size, which makes the decline of XRD diffraction peak. The photovoltaic performance of DSSCs was studied by using single-layer TiO₂ photoanode printed on different mesh number. It was found that the photovoltaic performances of DSSCs with 200 and 300 mesh were better than that of 400 mesh. The multilayer TiO₂ anode were printed using 100, 200, 300, and 400 mesh respectively, and the dye-sensitized solar cells were assembled. The results show that PCE for dye-sensitized solar cells has been significantly improved by the use of different mesh combination printing. It was the highest efficiency 6.9% under 300 mesh+200 mesh+100 mesh printing. The screen printing electrode preparation method without any chemical treatment, the printed film of high-mesh bottom layer is uniform and firm, the cell preparation has simple process, good repeatability, and the dye-sensitized solar cells have high PCE.