The treatment of bright white LED combined with classical music was proposed by laboratory mice. Mice suffered from chronic mild stress were divided randomly into three groups and exposed to 12:12 h bright light:dark (350 lx) conditions. The control group (C) received no treatment. The classical music group (M) was treated with classical music of 2 h per day. The classical music + bright light group (M+B) was treated daily with classical music of 2 h and 5 000 lx white LED light. Thirty days later, depression-like behaviors were assessed using saccharin solution preferences and forced swim tests. Mice in M and M+B demonstrated signi cantly shorter immobility times and longer climbing times and swimming times compared to those in C(p<0.001). It was observed that M+B experienced shorter immobility times (52.3±25.18 s) than M (78.9±14.82 s), and there was significant difference between two groups(t(9)=3.414, p=0.008<0.05). The combination therapy of bright white LED and classical music raised the vitality of mice and possessed antidepressant effect.