The absorption spectra of lutein in ethanol/water solution at different temperatures were measured by UV-Vis spectrophotometer and the spectral changes with time were studied. The results showed that in 1∶1 ethanol/water solution, the probability to form H-aggregate in two samples with different concentration (25.6 and 10.2 μmol·L-1) decreased with increasing temperature. In 1∶2 ethanol/water solution the stable aggregated structure didn’t change with temperature. Kinetic detection of absorption spectra of 1∶1 ethanol/water solution indicated that the absorption of H-aggregate increased exponentially as time went by. It was concluded from analysis that the hydrogen bonds of water play an important role in the formation of aggregates. The increase of water molecules and hydrogen in ethanol/water solution leads to the formation of stable aggregated structure.