The Yangtze River is the third largest river in the world and the longest and largest river in China. China has adopted a national strategy to protect the Yangtze River. A better understanding of the ecosystem services value along the Yangtze River would provide support for the Yangtze River protection strategy. Using Costanza’s method to estimate the ecosystem services value, the value of 10 ecosystem services was estimated within 1 km and 2 km from the Yangtze River in 2017. These 10 services were derived from the four established groupings of provisioning, regulating, supporting, and cultural services. This study compared and analyzed the changes in the ecosystem services value in the upper, middle, and lower reaches of the river, and in provinces, cities, and villages along the Yangtze River. The total ecosystem services value within 1 km and 2 km from the river was 37.208 and 43.769 billion yuan, respectively. Within 1 km, the ecosystem services value in the middle reaches was 12.93 billion yuan, while the next highest value was in the upper reaches at 12.45 billion yuan, and the downstream area had the smallest value of 11.855 billion yuan. Within 2 km, the value of upstream ecosystem services was the highest at 16.31 billion yuan, while the second highest value was in the middle reaches at 14.376 billion yuan, and the smallest value was in the downstream area at 13.083 billion yuan. In the Yangtze River Basin, regulating services played a leading role, accounting for 81.6% and 78.9% of the ecosystem services value within 1 km and 2 km from the river, respectively. Among the 10 ecosystem services, hydrological regulation was the most important, while the value of raw material production made the smallest contribution. Among the provinces and cities along the Yangtze River, the highest ecosystem services value was in Hubei Province, while the lowest values were in Shanghai and the Qinghai-Tibet Plateau. If villages within 1 km and 2 km from the river were to be relocated, the total regional ecological value would increase by 527 and 975 million yuan, respectively.