• Spectroscopy and Spectral Analysis
  • Vol. 29, Issue 5, 1304 (2009)
HUANG Wei1、*, PAN Jian-ji2, CHEN Rong1, LI Yong-zeng1, FENG Shang-yuan1, XIE Shu-sen1, and ZENG Hai-shan3
Author Affiliations
  • 1[in Chinese]
  • 2[in Chinese]
  • 3Cancer Imaging Department, BC Cancer Agency, Vancouver, CanadaV5Z 1L3
  • show less
    DOI: Cite this Article
    HUANG Wei, PAN Jian-ji, CHEN Rong, LI Yong-zeng, FENG Shang-yuan, XIE Shu-sen, ZENG Hai-shan. Measurement of Nasopharyngeal Carcinoma Tissue ex vivo by Raman Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2009, 29(5): 1304 Copy Citation Text show less

    Abstract

    Raman spectroscopy has shown its potential and advantages in detecting molecular changes associated with tissue pathology, which makes it possible to diagnose with optical methods non-invasively and real-time. A compact and rapid near-infrared (NIR)Raman system was developed using 785 nm diode laser, volume phase technology (VPT)holographic grating system and NIR intensified charge-coupled device (CCD)with a specially designed Raman fibre probe which can effectively reduce the interference of fluorescence and Rayleigh scattering, maximize the ability of Raman collection as well as correct the image aberration of a planar grating diffraction. Adopting this method, signal-to-noise ratio has been greatly improved and human tissue signals can be acquired in a short time. Raman signals from fat and musculature of fresh pork were measured and referenced for further optimization, then Raman spectra of nasopharyngeal carcinoma in vitro and the effect of storage time on them were measured in 1-5 s and discussed. The sensitivities and performance of the system will be further enhanced and more Raman data will be acquired and compared between normal and cancerous nasopharyngeal tissue, expecting to discover the statistical characteristics, which will benefit the diagnosis and treatment of early nasopharyngeal carcinoma or other tumors.
    HUANG Wei, PAN Jian-ji, CHEN Rong, LI Yong-zeng, FENG Shang-yuan, XIE Shu-sen, ZENG Hai-shan. Measurement of Nasopharyngeal Carcinoma Tissue ex vivo by Raman Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2009, 29(5): 1304
    Download Citation