Ordered noble metal nanostructured arrays have attracted much attention due to their unique optical properties and flexible tuning abilities, which have shown promising applications in variety of micro/nano optoelectronic devices. Among the numerous research works, fast fabrication of Au periodic nanostructures in large area is still one of the most popular issues. In this work, nanosphere self-assembling lithography techniques was employed for the fabrication of novel 2D Au nanostructure arrays with controlled Au particle sizes based on the monolayer periodic macroporous template. Moreover, the physical mechanism of the Au nanostructure formation was discussed in detail. The optical properties were investigated and the results clearly revealed the surface plasmon scattering and absorption properties of the Au nano array. Moreover, it was demonstrated with a remarkable two-photon absorption saturation effect for the 1 030 nm femtosecond pulses. The results imply the great potential of the Au nano arrays in many applications such as solar cell, optical switch and nano fabrication areas.