Micro-columnar structured γ-CuI scintillation conversion screen with columnar diameter in the micrometer and thickness about 17 μm were prepared by thermal evaporation method on quartz substrates with different temperatures.X-ray excited luminescence spectra of the screens show two peaks located at 430 nm and near 700 nm,which correspond to the fast and slow emission components,respectively.The fast one dominated.The intensity of 430 nm peak decreased as the substrate temperature rose from 170 ℃ to 210 ℃.At the same time the intensity of 700 nm band increased.The changes may be attributed to the iodine loss from screen caused by the substrate temperature.The phenomenon of iodine loss was observed by the Rutherford backscattering experiment.The crystal structure of the screens presents (111) preferred orientation,which is independent of the substrate temperature.As the temperature rose to 210 ℃,two weak additional peaks of (220) and (420) γ-CuI crystal planes in X-ray diffraction patterns appeared due to the increase in kinetic energy of CuI molecules.The scanning electron microscopy images of the screens showed that the columnar structure was improved when the substrate temperature increased from 170 ℃ to 190 ℃,but it would be degenerated when the temperature continued to rise to 210 ℃ because of the surface and bulk diffusion effects of the depositing molecules.Finally,the spatial resolution of the γ-CuI scintillation screens was measured by knife-edge method,and they are 4.5,7.2 and 5.6 lp·mm-1 for the screens prepared at the substrates temperatures of 170,190 and 210 ℃,respectively.The result shows that micro-column structure could improve the spatial resolution of γ-CuI scintillation screen.