The Al and Fe content in cement is one of the bases of classification and quality evaluation of cement. Laser-induced breakdown spectroscopy(LIBS) was used to quantitatively analyze the Al and Fe content in cement samples. According to the intensity of spectral lines and SNR，it was confirmed experimentally that the laser energy 30 mJ and delay time 1 μs were the best experimental parameters for single pulse laser induced breakdown spectroscopy to detect the Al and Fe in cement. Internal standard method was used to establish the calibration curve，the correlation coefficients of Al and Fe were 0.998 and 0.997. Using cycle inversion method to detect the accuracy of quantitatively analysis，it was found that the maximum relative error of Al and Fe were 2.32% and 5.11% while the average relative error were 1.34% and 2.40%. Experimental results show that the use of laser-induced breakdown spectroscopy on quantitative analysis of Al and Fe is feasible.