The canopy reflectance of rice was measured in the filed in order to monitor the damaged region caused by Cnaphalocrocis medinalis Guenee. The characteristics of canopy spectral reflectance were analyzed in contrast region and damaged regions. When rice plant was damaged by Cnaphalocrocis medinalis Guenee, the chlorophyll absorption was decreased in the band of 600-700 nm. The canopy reflectance of moderate damage region was lower than that of the contrast region, while the reflectance of severe damage region rice was higher near 550 nm. The canopy reflectance of Cnaphalocrocis medinalis Guenee damaged rice was fluctuant and exhibited the significant peak in the NIR band of 750-770nm. Meanwhile, red edge inflection point as one of the most important spectral parameters was analyzed at different damage levels based on the first derivative of reflectance spectra. The analysis results indicated that red edge inflection position moved to direction of blue light (short wavelength) with the affection severity increasing. Then the modified reflectance of rice canopy was calculated based on zero-mean calculation and standard deviation. It was easy to find the degree of deviation from the average of samples and distinguish the damaged region from experiment plots. The canopy modified reflectance was gently in the contrast region, but changed violently in the affected regions in the band of 750-950 nm. The analysis of Cnaphalocrocis medinalis Guenee affected regions illustrated that the Cnaphalocrocis medinalis Guenee was increased with the increase in severity. The vegetation index was applied in detection of Cnaphalocrocis medinalis Guenee damaged regions because of the composition of multi-wavelength information. The wavelengths 762 and 774 nm were chosen to build detection parameters of Cnaphalocrocis medinalis Guenee such as NIR-RVI, NIR-DVI, NIR-NDVI and KI. The results indicated that the NIR-NDVI could be used to identify the damaged region with contrast region efficiently. The accurate rate of 25 verification samples selected randomly reached 70%. The preliminary studies on rice Cnaphalocrocis medinalis Guenee damaged regions provided a new method to detect the affected regions in the wide area.