Infrared spectroscopy and dual-indicator sequence were used to study their IR characteristic peaks of ganoderma lucidums from different places and species. The experimental results showed that the majority of ganoderma lucidum from different places and species had the similar characteristic peaks of infrared spectra, Their common peak ratio could reach more than 91.67%, but the common peak ratio of mycelium was 52.94%-58.82%, and variant peak ratio was 29.41%-66.67%. The main variation peak was in the fingerprint regions 1 460.9-1 423.7 cm-1with ladder peaks. The IR characteristic peaks of ganoderma Lucidum obviously appear as the main characteristic peaks with the type of polysaccharide and protein bands. There was obviously a wide and strong absorption peak in 3 377.8-3 396.5 cm-1, a small acromion in 2 924.2-2 925.1 cm-1, a medium intensity absorption peak in 1 635.8-1 650.3 and 1 372.5-1 375.2 cm-1, a strong absorption bifurcate peak in 1 074.8-1 075.3 and 1 043.2-1 045.2 cm-1, a obvious weak peak in fingerprint regions 891.0-894.8 cm-1, and a medium intensity absorption peak in 563.10-574.7 cm-1.