Chlorophyll content reflects plant health status. Studying on spectral characteristics and regularity of tree leaves at different SPAD values can provide theoretical support for band recognition of chlorophyll hyperspectral monitoring and trees species health management. Nine tree species from shrub and arbor in Langya Mountain scenic spots were selected to explore the spectral characteristics of the same tree species with the SPAD values changing and the spectral differences of different tree species with the same SPAD values in this paper. At the same time, the relationship between different tree leaves chlorophyll content (SPAD) and the original spectrum, the inverse of the spectrum, the first order differential of the spectrum, the second order differential of the spectrum and the various band combinations of the spectrum such as difference index, normalization vegetation index, ratio index, first order differential normalization vegetation index, first order differential ratio index were discussed in depth. The results showed that the spectral characteristics of nine kinds of tree species leaves were distinct with the SPAD values changing, and the distinction was evident in the visible band, in general, the leaves SPAD values with the highest spectral reflectance was lower. In addition, the spectral reflectance of osmanthus leaves was higher than that of the other species in the visible band, and the spectral reflectance of Magnolia grandiflora leaves was always the first three in 780~1 350 nm band when the chlorophyll SPAD value was the same, but the changing law was not obvious in other bands. Moreover, the second order differential of the original spectrum had the highest correlation coefficient with the SPAD value of Pittosporum leaves, as the first order differential had the highest correlation with the others. The spectral indices with the highest correlation coefficient of SPAD value of shrubs and deciduous tree leaves were differential index and normalized vegetation index of first order differential of the spectrum, otherwise, evergreen trees and trees regardless of species were of the highest correlation coefficient with ratio index of the first order differential of the spectrum.