• Spectroscopy and Spectral Analysis
  • Vol. 33, Issue 4, 877 (2013)
YANG Xun*, LIU Xiao-chen, and MA Wan-yun
Author Affiliations
  • [in Chinese]
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    DOI: 10.3964/j.issn.1000-0593(2013)04-0877-04 Cite this Article
    YANG Xun, LIU Xiao-chen, MA Wan-yun. A Method for Real-Time Observation of Intracellular Free Ca2+ Concentration in Single Bovine Retinal Neurons[J]. Spectroscopy and Spectral Analysis, 2013, 33(4): 877 Copy Citation Text show less

    Abstract

    In order to observe the neurotrophin’s influence on intracellular free calcium concentration, the test neuron was labeled by fluorescence indicator Fluo-3, and imaged by self-built real-time fluorescence microscopy system. The authors observed the changes in intracellular free calcium concentration ([Ca2+]i) in the bovine retinal neurons under the influence of four kinds of neurotrophins such as brain derived neurotrophic factor BDNF. On account of the fact that fluorescence indicator’s intensity decays over time, it is necessary to apply a “decay removal correction” to the fluorescence intensity in order to show the fluorescence intensity that solely represents [Ca2+]i. The corrected data shows an increase after adding neurotrophins to neurons, which is consistent with similar results published by other groups. Thus, our method of imaging living cells is feasible, and “decay removal correction” is reliable.
    YANG Xun, LIU Xiao-chen, MA Wan-yun. A Method for Real-Time Observation of Intracellular Free Ca2+ Concentration in Single Bovine Retinal Neurons[J]. Spectroscopy and Spectral Analysis, 2013, 33(4): 877
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