Infrared focal plane arrays (IRFPAs) usually contain many defective pixels. These defective pixels have to be corrected because those can significantly impair the performance of infrared image of IRFPAs. As is known to all, infrared image acquisition and analysis based on Matlab can be helpful to identify and correct defective pixels. In this paper, we proposed a novel method to identify and correct defective pixels. In the phase of identification, the defective pixels could be identified by the algorithms combined with median filtering algorithm and improved standard deviation algorithm. In the phase of correction, proportion-spatial defective pixel replacement (PSDPR) algorithm was introduced to replace the defective pixels, and this method reduced the difficulty of replacement originating from the clustering phenomenon of defective pixels. In addition, an experiment of verification was done, and showed the proposed scheme worked effectively.