A set of direct current(DC) discharge device of N2 plasma was developed, carbon nanotubes(CNT) modified ITO electrode was used as anode, aluminum plate as cathode, with ~80 μm separation between them. Nitrogen emission spectra was observed at room temperature and low DC voltage (less than 150 V), and the emission spectrometry was used to diagnose the active species of the process of nitrogen discharge. Under DC discharge, the strongest energy band N2(C3Πu), the weak Gaydon’s Green system N2(H3Φu-G3Δg) and the emission line of nitrogen atoms (4p-4p0) at 820 nm were observed. Found that metastable state of nitrogen molecules were the main factors leading to a series of excited state nitrogen atoms and nitrogen ionization. Compared the emission spectra under DC with that under alternating current (AC) (1.1 kV), and it can be seen that under DC the spectra band of nitrogen atoms can be obviously observed, and there was a molecular band in the range of 500～800 nm. The effect of oxygen and hydrogen on the emission spectra of nitrogen was investigated. The results showed that the oxygen inhibited the luminescence intensity of nitrogen, but the shape of spectra unchanged. All of the second positive system, Gaydon’s Green system and atomic lines of nitrogen can be observed. The second positive system and Gaydon’s Green system of nitrogen will be greatly affected when the volume ratio of nitrogen and hydrogen greatly affected is 1∶1, which was due to the hydrogen. The hydrogen can depresse nitrogen plasma activation, and make the Gaydon’s Green System disappeared. CNT modified ITO electrode can reduce the breakdown voltage, and the optical signal generated by the weakly ionized gas can be observed by the photomultiplier tube at low voltage of 10 V.