Two-photon excitation fluorescence microscopy (TPM) is a powerful tool for the in vivo three-dimensional (3D) imaging of cellular and subcellular structures and functions deep in turbid tissues . Owing to its nonlinear excitation property, TPM provides compelling performance of near-diffraction-limited spatial resolution in relatively thick samples. However, the conventional TPM, i.e., Gaussian-focus TPM (Gauss-TPM), captures volumetric images by serially scanning a 3D space with a Gaussian focus, which significantly limits the imaging speed. Owing to its importance in capturing rapid biological events such as calcium transients in neurons, considerable effort has been devoted to improving the focus scanning rate [2,3], such as by applying resonant scanning , acoustic scanning [5–7], ultrasound lenses [8,9], electrotunable lenses [10,11], remote focusing [12,13], multifocal excitation [14–17], and multi-angle line scanning . Other strategies, including temporal focusing [19,20] and multiplane imaging , have been developed to increase the volumetric imaging speed by capturing a two-dimensional (2D) image within a single exposure. Although these methods can partially alleviate the problem by improving the scanning speed, the implementation of layer-by-layer scanning to visualize 3D structures inherently limits the volumetric imaging speed. Moreover, the layer-by-layer scanning strategy repeatedly exposes the tissues above or beneath the focal plane to the excitation light, thereby aggravating photodamage and phototoxicity.