Oocyte quality is the key factor to affect the ability of in-vitro fertilization, the potential of embryo development in vitro and the success of pregnancy of mammals, there by significantly affecting the assisted reproduction of human beings, the breeding of animals, the improvement of varieties and the preservation of endangered species. The research on oocyte quality has important application value. Currently, the common quality assessment methods are mainly performed from the morphology and biochemistry analysis perspective. However, the traditional method of evaluating the development quality of oocytes based on morphological selection has the characteristics of strong subjectivity, and the reliability of the results strongly depends on technicians’ experience. Although the new method of detecting biochemical indexes based on biological technology can make up for the shortcomings of morphological detection methods, there are still some shortcomings of invasive research, complicated biological operation steps, long experimental time-consuming and affecting the subsequent development of oocytes. As we all know, Abnormal changes in biological tissue are often accompanied by changes in its internal biochemical components, which often shows spectral differences. Thus, this paper used ultraviolet-visible (UV-Vis) spectrophotometer and self-modified multispectral imaging system to study the spectra of oocytes, and the spectral data were analyzed between immature and mature oocytes, fresh mature and postovulatory aged oocytes. It was found that (1) compared with the UV-Vis spectra (190~1 100 nm) of fresh matured oocytes, the spectra of immature oocytes and aged oocytes showed several peak changes. The UV-Vis spectra of immature oocytes missed two peaks of 205 and 579 nm and the band of 894~941 nm and added the peak of 593nm compared with that of fresh matured oocytes. For the UV-Vis spectra of the aged oocytes, three peaks of 205, 445 and 579 nm and the band of 846 to 941 nm disappeared and the peak at 593 nm also increased. (2) The visible-band multispectral imaging data showed that compared with fresh matured oocytes, the transmission spectrum intensity at various biological structures of immature oocytes decreased at 451 and 467 nm, and the corresponding data of aged oocytes decreased at 425, 431, 571 and 669 nm. In summary (specially, the spectral differences between fresh matured and aged oocytes in multispectral imaging)， it is thus clear that spectral detection shows great feasibility in identifying oocyte quality.